The effect of lactic acid bacteria addition on microbiota and occurrence of mycotoxins in rye silages

In the rye silages (control and with additive) microbiota isolation identification (total count of bacteria, coliform enterococci, lactic acid bacteria microscopic filamentous fungi) occurrence mycotoxins were evaluated. Total on Plate agar at 30 °C for 48-72 hours, McConkey 37 24-48 enterococci Enterococcus selective De Man, Rogosa Sharpe agar, Mayeux, Sandine Elliker All Purpose TWEEN® hours in microaerophilic condition fungi Malt extract 25 7 days evaluated identified mass spectrometry. The most isolated family from both types silage was Lactobacillaceae. species control group Lentilactobacillus buchneri (22%) lowest number Mucor spp. additive Lactobacillus gasseri (16%). ELISA method (enzyme-linked immunosorbent assay) using reader wavelength 650 nm detection quantification aflatoxins, total ochratoxins, fumonisins, deoxynivalenol, zearalenone T-2/HT-2 toxin) used. characterized by all analyzed mycotoxins, whereas deoxynivalenol mycotoxin highest concentration. had significantly lower content aflatoxins compared to control, while higher concentration toxin determined.